Bacterial Genomic Reorganization upon DNA Replication

Bacterial genomic reorganization upon DNA replication.

Reorganization upon DNA Replication Myllykallio et al. (1) narrowed the replication origin of the archaeon Pyrococcus abyssi down to an 80-kb region (bar on left axis of Fig. 1A), an observation consistent with our prediction (2) of the replication origin of a closely related species, Pyrococcus OT3 (triangle on upper axis of Fig. 1A). Here, we report that a comparison of the genomic sequences ...

Bacterial Genomic Reorganization upon DNA Replication

Reorganization upon DNA Replication Myllykallio et al. (1) narrowed the replication origin of the archaeon Pyrococcus abyssi down to an 80-kb region (bar on left axis of Fig. 1A), an observation consistent with our prediction (2) of the replication origin of a closely related species, Pyrococcus OT3 (triangle on upper axis of Fig. 1A). Here, we report that a comparison of the genomic sequences ...

Hemicatenanes form upon inhibition of DNA replication.

Plasmid DNA incubated in interphase Xenopus egg extracts is normally assembled into chromatin and then into synthetic nuclei which undergo one round of regulated replication. During a study of restriction endonuclease cut plasmid replication intermediates (RIs) by the Brewer-Fangman 2D gel electrophoresis technique, we have observed the formation of a strong spike of X-shaped DNA molecules in e...

Tumour suppressor ING1b maintains genomic stability upon replication stress

The lesion bypass pathway, which is regulated by monoubiquitination of proliferating cell nuclear antigen (PCNA), is essential for resolving replication stalling due to DNA lesions. This process is important for preventing genomic instability and cancer development. Previously, it was shown that cells deficient in tumour suppressor p33ING1 (ING1b) are hypersensitive to DNA damaging agents via u...

Initiation of DNA Replication: The Genomic Context